基于uC/OS-II的微流体芯片嵌入式实时系统构建

本文构建具有DNA 聚合酶链式反应（PCR）和毛细管电泳(CE)分离检测功能的微流体芯片uC/OS-II嵌入式实时控制系统。本系统采用32 位嵌入式微控制器ARM 实现PCR 扩增所需的闭环温度控制功能，毛细管电泳分离功能所需的高压电场自动调度功能。uC/OS-II 嵌入式实时操作系统加强了系统的实时性。模糊免疫PID 控制算法实现对温度的精确控制，其控制性能远优于常规PID 控制器。试验结果表明本系统不但完全满足设计要求，而且与同功能的仪器平台相比，实时性强，不再需要昂贵的个人电脑进行控制运算和常用的DNA 检测设备进行检测，实现了PCR-CE 微流体芯片系统的低成本和小型化。

关键词：微流体芯片；ARM； 模糊免疫PID 算法

Real Time Embedded System in Micro-fluidic Chips Based on the uC/OS-II kernel WANG-haifeng 1 ，ZHANG-haili 2 ， ZHANG -yulin1 (1.School of Control Science and Engineering ，Shandong University，250061，Jinan, China; 2.Automation Lab , Shandong Institute of Light Industry, 250100，Jinan, China) Abstract This paper has developed integrated polymerase chain reaction - capillary electrophoresis (PCR-CE) micro-fluidic chips real-time embedded system based on uC/OS-II, can perform a series of analytical steps ranging from sample pre-concentration, PCR and CE separation. The miniaturized device platform has been designed based on the embedded, 32 bites RISC microcontroller ARM. Because the ability to precisely control temperature is a critical component of PCR micro-fluidic devices, the fuzzy immune PID control arithmetic is used to ensure the temperature control precise and robust. The platform includes matrix

keyboard, small LCD displayer, serial communication unit, electroosmosis flow unit and

PCR unit,. The miniaturized device platform can not only real-time fulfill all the functions

in the PCR-CE micro- fluidic chips without the help of expensive personal computers and

other measure devices but also realize the micro-fluidic chips system low cost and

miniaturization.

Keywords: Micro-fluidic chip ; uC/OS-II; ARM ; Fuzzy immune PID